Coding

Part:BBa_K4378003:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)

Leaf Compost Cutinase with 6xHis-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

During the design of this part, one internal EcoRI site was mutated out in silico prior to synthesis. Additionally this part has been codon optimized for E. coli.

Source

Sequence originates from a metagenomic analysis of leaf-branch compost, the sequence published here was adapted from the following publication:

Sulaiman S, Yamato S, Kanaya E, Kim J-J, Koga Y, Takano K, Kanaya S. 2012. Isolation of a novel cutinase homolog with polyethylene terephthalate-degrading activity from leaf-branch compost by using a metagenomic approach. Applied and Environmental Microbiology 78: 1556–1562.